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c proceed  (New England Biolabs)


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    Structured Review

    New England Biolabs c proceed
    C Proceed, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 449 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+proceed/NEBNext+Ultra+Ligation+Module/us12410471-246-27-31
    Average 95 stars, based on 449 article reviews
    c proceed - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Ligation:

    Article Title: DNA construct for sequencing and method for preparing the same
    Article Snippet: End Repair/dA-Tailing Follow the NEBNext® UltraTM End Repair/dA-Tailing Module (NEB #E7442) protocol: Mix the following components in a sterile, nuclease-free tube: (green) End Prep Enzyme Mix—3.0 μl; (green) End Repair Reaction Buffer (10×)—6.5 μl; PCR amplicons from previous step—55.5 μl; Mix by pipetting, followed by a quick spin to collect all liquid from the sides of the tube. .. Place in a thermocycler, with the heated lid on, and run the following program: 30 minutes @ 20° C.; 30 minutes @ 65° C.; Hold at 4° C. Proceed directly to NEBNext Ultra Ligation Module (NEB #E7445): If DNA input prior to End Repair is <100 ng, dilute the xGen® Dual Index UMI Adapters 1:10 in 10 mM Tris-HCl pH 7.5-8.0 or 10 mM Tris-HCl pH 7.5-8.0 with 10 mM NaCl to a final concentration of 1.5 μM. ..

    Concentration Assay:

    Article Title: DNA construct for sequencing and method for preparing the same
    Article Snippet: End Repair/dA-Tailing Follow the NEBNext® UltraTM End Repair/dA-Tailing Module (NEB #E7442) protocol: Mix the following components in a sterile, nuclease-free tube: (green) End Prep Enzyme Mix—3.0 μl; (green) End Repair Reaction Buffer (10×)—6.5 μl; PCR amplicons from previous step—55.5 μl; Mix by pipetting, followed by a quick spin to collect all liquid from the sides of the tube. .. Place in a thermocycler, with the heated lid on, and run the following program: 30 minutes @ 20° C.; 30 minutes @ 65° C.; Hold at 4° C. Proceed directly to NEBNext Ultra Ligation Module (NEB #E7445): If DNA input prior to End Repair is <100 ng, dilute the xGen® Dual Index UMI Adapters 1:10 in 10 mM Tris-HCl pH 7.5-8.0 or 10 mM Tris-HCl pH 7.5-8.0 with 10 mM NaCl to a final concentration of 1.5 μM. ..



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